analysis of phenolic compounds in matricaria chamomilla and its extracts by uplc-uv
Authors
abstract
chamomile ( matricaria chamomilla l.) is a widely used medicinal plant possessing several pharmacological effects due to presence of active compounds. this study describes a method of using ultra performance liquid chromatography (uplc) coupled with photodiode array (pda) detector for the separation of phenolic compounds in m. chamomilla and its crude extracts. separation was conducted on c 18 column (150 mm × 2 mm, 1.8 µm) using a gradient elution with a mobile phase consisting of acetonitrile and 4% aqueous acetic acid at 25°c. the method proposed was validated for determination of free and total apigenin and apigenin 7-glucoside contents as bioactive compounds in the extracts by testing sensitivity, linearity, precision and recovery. in general, uplc produced significant improvements in method sensitivity, speed and resolution. extraction was performed with methanol, 70% aqueous ethanol and water solvents. total phenolic and total flavonoid contents ranged from 1.77 to 50.75 gram (g) of gallic acid equivalent (gae)/100 g and 0.82 to 36.75 g quercetin equivalent (qe)/100 g in dry material, respectively. there was a considerable difference from 40 to 740 mg/100 g for apigenin and 210 to 1110 mg/100 g for apigenin 7-glucoside in dry material.
similar resources
Analysis of phenolic compounds in Matricaria chamomilla and its extracts by UPLC-UV
Chamomile (Matricaria chamomilla L.) is a widely used medicinal plant possessing several pharmacological effects due to presence of active compounds. This study describes a method of using ultra performance liquid chromatography (UPLC) coupled with photodiode array (PDA) detector for the separation of phenolic compounds in M. chamomilla and its crude extracts. Separation was conducted on C18 co...
full textNanoparticles induced antioxidative compounds in Matricaria chamomilla
In this study, the effects of different concentrations of silica nanoparticles (NPs) were studied on growth, membrane stability, and antioxidant properties of Matricaria chamomilla in vitro. The sterilized seeds were incubated in different concentrations of silica NPs (0, 2, 4, 6 g L-1<span st...
full textAntioxidant properties and quantitative UPLC-MS/MS analysis of phenolic compounds in dandelion (Taraxacum officinale) root extracts
Prolonged exposure to metabolically derived reactive oxygen species (ROS) can cause an imbalance between radical species and in vivo physiological antioxidant defences. As a result, ROS have the potential to bind and irreversibly oxidise nucleic acids, proteins, lipids, carbohydrates and DNA.1 Failure to repair or remove these oxidised molecules may lead to cellular dysfunction and mutation, wh...
full textextraction and characterization of allium irancum plant extract and its application in the green synthesis of silver nano particles and oxidation of thiocarbony1 compounds
سنتز سبز نانوذرات فلزی (nps) درسالهای اخیر توجه بسیارزیادی را به خود جلب کرده است. زیرا این پروتوکل کم هزینه وسازگار با محیط زیست از روش های استاندارد سنتز. در این پایان نامه ما گزارش میکنیم یک روش ساده و سازگار با محیط زیست برای سنتز نانوذرات نقره با استفاده از محلول آبی عصاره گیاه allium iranicum به عنوان یک عامل کاهش دهنده ی طبیعی. نانو ذرات نقره مشخص شد با استفاده از تکنیک های uv-visible، x...
Matricaria chamomilla CH12 decreases handling stress in Nelore calves
Matricaria chamomilla CH12 is a phytotherapeutic or homeopathic product, which has been used to reduce stress. Here, we examined its effect on preventing handling stress in bovines. Sixty Nelore calves were randomly distributed into two equal groups. One group was administered Matricaria chamomilla CH12 in diet and the other the 'control' was not. Animals in both groups were maintained unstress...
full textMy Resources
Save resource for easier access later
Journal title:
research in pharmaceutical sciencesجلد ۹، شماره ۱، صفحات ۳۱-۰
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023